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max in WT synaptoneurosomes, suggesting that Src signaling might be downregulated in KI synapses. However, our ability to rescue SERT functionality in KI midbrain synaptoneurosomes because of the inhibition of FAK suggests elevated FAK signaling downstream from the Pro32Pro33 mutant, as verified by greater pFAK localization in 5-HT synapses. Our information therefore advise that the Src–FAK pathway is uncoupled by constitutive activation of integrin βthree, and thus Every kinase may be influencing SERT independently.
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